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chem331:bioassay_results

JMD Assay Results

Natural Set Plate:13091250

Data Analysis of Possible FabG Inhibitors

Potential FabG Inhibitors

7-Hydroxyphenoxazin-3-one

WellID: G3

3-Benzofurancarboxylic acid, 2-(2,4-dihydroxyphenyl)-6-hydroxy-, .delta.-lactone

WellID: F4

10-Hexadecenoic acid, 5-[[4-(diemthylamino)tetrahydro-3-hydroxy-6-methyl-5-[(tetrahydro-4,5-dihydroxy-4,6-dimethylpyran-2-yl)oxy]-pyran-2-yl]oxy]-12,13-epoxy-6-(formylmethyl)-3,15-dihydroxy-4-methoxy-8-methyl-9-oxo-,.omega.-lactone, acetate, isovalerate

WellID: E7

beta-Lapachone

WellID: C5

Natural Set Plate:13091251

Due to improperly dissolved chemicals, the assay results were inconsistent, and possible hits could not be determined.

BMS Assay Results

Potential Inhibitors:

2-(3-hydroxycyclohexyl)-6,7-dimethyl-dodecahydrofuro[2,3-b]quinoxalin-3-ol(A11)

2-(2-methoxyphenyl)-7-nitro-2H,4H-furo[2,3- b]quinoxalin-3-ol(B11)

Syringil(D11)

1,8-diphenyl naphthalene-1,8-dicarboxylate(E11)

6-bromo-n-naphthalen-1-yl-1_3-benzodioxole-5-carboxamide (G9)

Potential Inhibitors:

N-[4-(pyridine-4-amido)phenyl]pyridine-4- carboxamide (F2)

1-hydroxy-4-{[4-(2-hydroxyethyl)phenyl]amino}anthracene-9,10-dione (H5)

JDC Assay Results

Diversity Plate #4729 Results

Bioassay Results (Plate # 4729)

The bioassay of plate 4729 resulted in the finding of two possible fabG inhibitors. The fabG inhibition was indicated by the comparison of growth between the control strain after 24 hours and the fabG-overexpressed strain after 24 hours. From the bar graphs one sees what a “hit” entails: a significant percent change in growth where there was higher inhibition for the control strain than the fabG strain.

The first possible fabG inhibitor was found in well E10 with NSC number 45291. Its molecular formula is C12H10N2S and its structure is seen below.

Structure of C12H10N2S

The second possible fabG inhibitor was found in well E3 with NSC number 79582. The name of this compound Benzo[c]cinnoline, N-oxide with molecular formula C12H8N2O. Its structure is also seen below.

Structure of Benzo[c]cinnoline, N-oxide

Natural Products Plate #13091251 Results

Bioassay Results (Plate # 3891)

The same bioassay protocol was followed for the bioassay of natural products plate 13091251, which resulted in the finding of four possible fabG inhibitors.

The first possible fabG inhibitor was found in well C4 with NSC number 267461. The name of this compound is Nanaomycin A and its molecular formula is C16H14O6 and its structure is seen below.

Structure of Nanaomycin A

The second possible fabG inhibitor was found in well G5 with NSC number 79582. The name of this compound Propanamide, 2-amino-N-[3-(dichloromethyl)-3,4,4a,5,6,7-hexahydro-5,6,8-trihydroxy-3-methyl-1-oxo-1H-2-benzopyran-4-yl]-, [3S-[3.alpha.,4.alpha.(R*),4a.beta.,5.beta.,6.alpha.]]- with molecular formula C14H20Cl2N2O6. Its structure is also seen below.

Structure of C14H20Cl2N2O6

The third possible fabG inhibitor was found in well G8 with NSC number 526417. Its molecular formula is C51H64N12O12S2. Its structure is seen below.

Structure of C51H64N12O12S2

The fourth possible fabG inhibitor was found in well G10 with NSC number 400978. This molecule is called Spiro[cyclopropane-1,5'-[5H]inden]-7'(6'H)-one, 2',3'-dihydro-3',6'-dihydroxy-2',2',4',6'-tetramethyl-,(3'S-trans)- and its molecular formula is C15H20O3. Its structure is seen below.

Structure of C15H20O3

by Jess Coulter, Daniel Rossie, & Cameron Kubota

chem331/bioassay_results.txt · Last modified: 2016/06/07 09:53 (external edit)