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Chem 331 Advanced Cellular Biochemistry Wiki

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Arsenate DNA

Project Summary

This purpose of this project is to introduce students to critical reading and analysis of scientific literature. The context for this project is the controversial 2010 report in Science of a bacterium that was able to substitute arsenate for phosphate in its DNA. Students will read the original paper as well as the responses to the paper from the scientific community that were published in Science, and then write an in-depth wiki article that focuses on a specific topic from the paper.


DNA Replication

Project Summary

The purpose of this project is for students to write an in-depth article about a specific enzyme that participates in DNA replication.


FabG Antibacterial Bioassay

Project Summary

Drug-resistance in pathological bacteria is a rapidly growing problem in Western societies. Therefore, the discovery of new chemical compounds with antibacterial activity is a critical problem for modern science to solve. In our lab activities this semester, we will attempt to create a bioassay that will enable us to search for chemical compounds with antibacterial biological activities. Our specific molecular target will be the FabG protein from Escherichia coli.

Project Background

Fatty acids are essential molecules for living organisms. Bacteria use a different type of enzymes (type II FAS) than mammals (including humans) (type I FAS) to synthesis fatty acids. Therefore, inhibition of these type II FAS enzymes provides an opportunity to inhibit bacterial growth and viability without effecting human enzymatic processes. This combination makes the type II FAS enzymes favorable targets for antimicrobial research. Many bacteria use a series of type II FAS enzymes named the FabX proteins (where X = A, B, C, D, etc.). In particular FabF, FabH and FabI have all been proposed as ideal targets for antibacterial drugs. Related to these, the FabG enzyme has also been identified as a possible drug target, but it has not received as much attention as the other FabX enzymes. FabG catalyzes the formation of β-ketoacyl-ACP intermediates in the FAS pathway via an NADH dependent reduction. We will use FabG as the primary target of our antibacterial drug discovery project.


chem331/chem_331_wiki_projects.txt · Last modified: 2016/06/07 09:53 (external edit)